Journal: NPJ Breast Cancer

Article Title: Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers

doi: 10.1038/s41523-021-00350-5

Figure Lengend Snippet: a Western blot showing MRE11, RAD50 and NBS1 protein expression in a panel of breast cancer cell lines. All blots derive from the same experiment and they were processed in parallel. b Photomicrographs showing immunohistochemical staining of MRE11, RAD50 and NBS1 in breast cancers. c Correlation Matrix showing the correlation between levels of MRE11, NBS1 and RAD50 protein expressions and other DNA repair biomarkers. Blue colour refers to positive (+) correlation, while the red colour reflects negative (−) correlations. The size of the circles and intensity of the colour propionate to the correlation coefficient. The image was generated using RStudio software. Red arrow = proteins investigated in the current study i.e. MRE11, RAD50 and NBS1.

Article Snippet: A further set of slides were incubated for 18 h at 4 °C with the primary mouse monoclonal anti-RAD50 antibody (Ab489, Abcam), at a dilution of 1:100.

Techniques: Western Blot, Expressing, Immunohistochemical staining, Staining, Generated, Software

Journal: NPJ Breast Cancer

Article Title: Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers

doi: 10.1038/s41523-021-00350-5

Figure Lengend Snippet: MRN complex protein expression and clinicopathological features in breast cancer.

Article Snippet: A further set of slides were incubated for 18 h at 4 °C with the primary mouse monoclonal anti-RAD50 antibody (Ab489, Abcam), at a dilution of 1:100.

Techniques: Expressing, Over Expression

Journal: NPJ Breast Cancer

Article Title: Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers

doi: 10.1038/s41523-021-00350-5

Figure Lengend Snippet: a Kaplan–Meier curve for MRE11 nuclear protein expression and breast cancer-specific survival (BCSS) in the whole cohort. b Kaplan–-Meier curve for MRE11 nuclear protein expression and BCSS in ER+ cohort. c Kaplan–Meier curve for MRE11 nuclear protein expression and BCSS in ER− cohort. d Kaplan–Meier curve for RAD50 nuclear protein expression and BCSS in the whole cohort. e Kaplan–Meier curve for RAD50 nuclear protein expression and BCSS in ER+ cohort. f Kaplan–Meier curve for RAD50 nuclear protein expression and BCSS in ER− cohort. g Kaplan–Meier curve for NBS1 nuclear protein expression and BCSS in the whole cohort. h Kaplan–Meier curve for NBS1 nuclear protein expression and BCSS in ER+ cohort. i Kaplan–Meier curve for NBS1 nuclear protein expression and BCSS in ER− cohort.

Article Snippet: A further set of slides were incubated for 18 h at 4 °C with the primary mouse monoclonal anti-RAD50 antibody (Ab489, Abcam), at a dilution of 1:100.

Techniques: Expressing

Journal: NPJ Breast Cancer

Article Title: Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers

doi: 10.1038/s41523-021-00350-5

Figure Lengend Snippet: a Kaplan–-Meier curve for RAD50 mRNA expression and breast cancer-specific survival (BCSS) in the whole cohort. b Kaplan–Meier curve for RAD50 mRNA expression and BCSS in ER+ cohort. c Kaplan–Meier curve for RAD50 mRNA expression and BCSS in ER− cohort. d Kaplan–Meier curve for has-miR-99b expression and BCSS in the whole cohort. e Kaplan–Meier curve for has-miR-99b expression and BCSS in ER+ cohort. f Kaplan–Meier curves for has-miR-99b expression and BCSS in ER− cohort. g Kaplan–Meier curves for has-miR-494 expression and BCSS in the whole cohort. h Kaplan–Meier curve for has-miR-494 expression and BCSS in ER+ cohort. i Kaplan–Meier curve for has-miR-494 expression and BCSS in ER− cohort.

Article Snippet: A further set of slides were incubated for 18 h at 4 °C with the primary mouse monoclonal anti-RAD50 antibody (Ab489, Abcam), at a dilution of 1:100.

Techniques: Expressing

Journal: NPJ Breast Cancer

Article Title: Untangling the clinicopathological significance of MRE11-RAD50-NBS1 complex in sporadic breast cancers

doi: 10.1038/s41523-021-00350-5

Figure Lengend Snippet: Differentially expressed genes (DEGs) were identified using RNAseq data from the TCGA-BRCA cohort dichotomized on the basis of low (quartile 1) versus high (quartile 4) expression of MRE11 ( a ) , RAD50 ( b ) and NBN/NBS1 ( c ). We next identified DEGs that were low (77 genes) or higher (545 genes) in patients with lower expression of MRN components ( d – f ) and identified enriched KEGG pathways ( d , e ). Pathways related to mitochondrial dysfunction were associated with reduced MRN expression ( f ).

Article Snippet: A further set of slides were incubated for 18 h at 4 °C with the primary mouse monoclonal anti-RAD50 antibody (Ab489, Abcam), at a dilution of 1:100.

Techniques: Expressing

Journal: Oncogene

Article Title: PML NBs associate with the hMre11 complex and p53 at sites of irradiation induced DNA damage.

doi: 10.1038/sj.onc.1205227

Figure Lengend Snippet: Figure 5 PML: p53 and p53: hMre11 complex associates with sites of double-strand breaks; (A) Immunostaining of PML (blue ¯uorescence, a ± f), p53 (red ¯uorescence, g ± n), g-H2AX (green ¯uorescence, o ± t); in MERGE (u ± k) the extent of colocalization among the three proteins is visualized: yellow staining indicates the presence of a binary complex (p53:g-H2AX); white staining reveals the association of the three proteins. (B) hRad50: p53: g-H2AX colocalization at 12 h after irradiation; in `Merge' colocalization of the three proteins is shown in white

Article Snippet: The following primary antibodies were used: anti PML (monoclonal PGM3 or polyclonal anti-Myl), goat anti p53 FL-393 (Santa Cruz Biotechnology), rabbit polyclonal anti hMre11 (Novus Biological), rabbit polyclonal anti gH2AX (Oncogene Science), monoclonal anti hRad50 (Novus Biological).

Techniques: Immunostaining, Staining, Irradiation